Biotin is required for the fatty acids and glucose formation so that essential energy is produced. Biotin also assists in the metabolism of fats, proteins and carbohydrates. Biotin is available in food naturally and the dietary sources of biotin comprise of brewer’s yeast, cauliflower, bananas, egg yolks, salmon, liver, sardines, mushrooms and legumes. The deficiency of biotin is uncommon. The other possibilities for having biotin as deficiency is genetic biotin deficiency, surgical stomach removal, excessive consumption of alcohol and infant dermatitis. Biotin deficiency leads to hair loss, skin rash, heart problems and high cholesterol. Biotin supplements are effective for eczema, acne, diaper rash, brittle nails and hair loss. People having a history of seizures should avoid using biotin. This is a B vitamin that helps in converting food to energy. It is mandatory that minimum 30 mg is taken daily as there are adequate health benefits. Normal biotin intake strengthens fingernails and also controls blood sugar levels.

Chromium.

L-Isoleucine- The second of three branched chain amino acids it is important in blood sugar regulation and producing hemoglobin. After exercise, it is responsible for muscle recovery and important in the metabolism to produce energy.

L-Leucine- Leucine is an essential amino acid that does not synthesize in humans and so must be ingested. It slows the degradation of muscle tissue and is used in the formation of sterols.

L-Valine is an essential alpha amino acid and one of the three essential branched chain amino acids found in the human body. The other two essential BCAAs are leucine and isoleucine. The name is derived from the plant valerian. Valine is also classified as nonpolar and is known by sickle cell disease sufferers as the substitute amino acid for the glutamic acid, whereby the hydrophobic qualities of valine cause the hemoglobin to not fold correctly. Just like the other three BCAAs, the primary source of valine is through ingestion of proteins, where valine is a component; some such sources would be fish, peanuts, poultry, cottage cheese, lentils and sesame seeds. L-Valine is found in large quantities in muscles, whereby in the muscle metabolism it is essential for the restoration and tissue growth.

Vitamin B12, also known as cobalamin, which is naturally found in foods such as fish, milk and milk products, eggs, meat and poultry. Vitamin B12 is important for good health, necessary for the proper digestion and absorption of foods and for normal metabolism of carbohydrates, and fat. It helps maintain healthy nerve cells and red blood cells and is needed to make DNA. Vitamin B12 is bound to the protein in food. Hydrochloric acid in the stomach releases B12 from protein during digestion. Unlike other water-soluble nutrients, vitamin B12 is stored in the liver, kidney, and other body tissues. It can take several years before signs of the deficiency appear due to poor dietary intake. Vitamin B12 functions as a "methyl donor." A methyl donor is a compound that carries and donates methyl groups to other molecules, including cell membrane components and neurotransmitters. As a methyl donor vitamin B12 is involved in homocysteine metabolism and plays a critical role in proper energy metabolism, immune function, and nerve function. Therefore, vitamin B12 is clearly an added benefit for anyone wanting to lose weight, as without it, metabolism is not at its peak potential.

Vitamin B6contains pyridoxal phosphate, or PLP for short, as the active ingredient and is a vitamin belonging to the B complex grouping. It is functioning mainly as a co-enzyme with others in the human body, aiding in the proper function of several metabolic processes. Neurotransmitter synthesis, as well as hemoglobin and histamine synthesis are just some of the functions of PLP, hemoglobin function, gene expression and aiding in the facilitation of decarboxylation, elimination and other reactions, mainly taking place in the liver. Vitamin B6 was found to diminish by 50% the risk of Parkinson’s disease. Alternative medicine suggests the use of Vitamin B6 to combat autism, in combination with magnesium. This combination has shown effects with children suffering from attention deficit disorders. PLP also seems to show therapeutic effects for diabetic nephropathy.

Zinc is an essential mineral that is used in many healing processes to speed up the healing process in instance that include sunburned skin, diaper rash and helps prevent premature aging of the skin.