Biotin is essential to cellular metabolism, as it plays an important coenzyme role in the metabolism of fats, carbohydrates and proteins.

Chromium.

L-Isoleucine is an essential alpha-amino acid that is considered both, a ketogenic and a glucogenic amino acid. Since that L-Isoleucine is an essential amino acid, it cannot be synthesized by the body, therefore it needs to be ingested. The primary source of L-Isoleucine is as a component of proteins. It has become a prominent component of nutritional supplements, mostly with the body building and similar athletic communities, where it is believed that L-Isoleucine has the capability to increase endurance, but also to repair muscle tissue. It has been observed that L-Isoleucine ingestion allows power athletes to recover easier from very tough trainings and exhausting activities. This branched chain amino acid is one of the three main BCAA in the body, the other ones being valine and leucine.

L-Leucine with the food additive E number code E641 is classified as a flavor enhancer. Leucine is actually an essential alpha-amino acid and a vital part of so called “buffer” proteins, such as ferritin and astacin. You can find leucine in foods such as whole wheat bread products, nuts and brown rice. As a supplement, researchers have found that leucine slows the degradation of muscles and muscle tissues through an inflated synthesis of muscle proteins. In the human body, leucine can be found in the liver, but most prominently in the muscle and adipose tissues, where it is utilized in production of sterols. In body building circles L-Leucine as a dietary supplement is very popular for all these properties, but also for the preservation of muscle glycogen, nitrogen balance maintenance and more.

L-Valine is the third branched chain amino acid and is required for muscle metabolism, repairs and regenerates tissue, and maintains the body's nitrogen level. It also is used as a muscle energy source and preserves the use of glucose.

Vitamin B12 is a water-souble member of the vitamin B complex. Requirements are in the microgram range, which is lower than the milligram range of the other B vitamins. Vitamin B12 is involved in the production of red blood cells and is usually known as the anti-anemia vitamin.

Vitamin B6, which active form pyridoxal phosphate or otherwise known as PLP and pyridoxal-5’-phosphate, is a prosthetic group of tightly bound cofactors responsible for many reactions within the amino acid metabolism and the manipulation of the enzymatic reaction behind the release of glucose from glycogen. Vitamin B6 generally comprises three organic compounds, pyridoxine, pyridoxal and pyridoxamine, all of them heterocyclic organic compounds that are converted in the human body to the active pyridoxal phosphate form. There are three reactions attributed to PLP, namely transamination, alpha elimination and beta elimination. Over one hundred and forty enzyme activities depend on pyridoxal phosphate, neurotransmitter and histamine synthesis, synthesis and function of hemoglobin, and gene expression. As a cofactor, PLP is active in amino acid metabolism, glucogenesis and lipid metabolism.

Zinc is a mineral that is present in every cell in the body. It is good for maintaining a healthy skin. It promotes healing of wounds such as bed sores, skin sore, and canker sores. It may also act as an astringent and aid in the treatment of acne. Zinc improves the immune system by activating white blood cells to fight against infections, virus, and even cancers. Zinc is also beneficial in improving the health of prostate gland and protects it against inflammation and infection. It also helps improve the brain functions such as memory retention. It also helps in the production of testosterone that improves libido, prevents low sperm count. It may also help in the formation and development of muscles.