Biotin is essential to cellular metabolism, as it plays an important coenzyme role in the metabolism of fats, carbohydrates and proteins.

Chromium.

L-Isoleucine is an essential alpha-amino acid that is considered both, a ketogenic and a glucogenic amino acid. Since that L-Isoleucine is an essential amino acid, it cannot be synthesized by the body, therefore it needs to be ingested. The primary source of L-Isoleucine is as a component of proteins. It has become a prominent component of nutritional supplements, mostly with the body building and similar athletic communities, where it is believed that L-Isoleucine has the capability to increase endurance, but also to repair muscle tissue. It has been observed that L-Isoleucine ingestion allows power athletes to recover easier from very tough trainings and exhausting activities. This branched chain amino acid is one of the three main BCAA in the body, the other ones being valine and leucine.

L-Leucine is not prepared in the body but can be obtained through food items like brown rice, whole wheat bread and nuts or dietary supplements. It provides power and endurance. It ensures the well being of the lean muscle tissue, maintains the body’s nitrogen balance and supplies energy especially during exercise. It also assists the healing process and preserves and builds muscles. It is ideal for sportspersons. L-Leucine is also known to increase the secretion of Human Growth Hormone resulting in healthy joints, the reduction of body fat and slowing down of the aging process. It is also useful to people with Diabetes and kidney and liver problems. In the case of a deficiency, the body will not be able to use any proteins efficiently.

L-Valine is the third branched chain amino acid and is required for muscle metabolism, repairs and regenerates tissue, and maintains the body's nitrogen level. It also is used as a muscle energy source and preserves the use of glucose.

Vitamin B12 is required for the normal functioning and development of the nervous system, blood cells and brain. It’s a water soluble vitamin which is used to elevate mood, improve energy levels and concentration, to boost immune system and in sleeping disorders. Severe deficiency of Vitamin B12 can lead to pernicious anemia. It is also used for lowering high homocysteine levels which may contribute to heart disease, asthma, AIDS, to prevent cervical and other cancers, memory loss, osteoporosis, allergies and skin infections. It also helps to prevent atherosclerosis, multiple sclerosis, and kidney and gum disease. Vitamin B12 is added in almost all multi-vitamin B complex supplements. Deficiency of Vitamin B12 in infants can lead to megaloblastic anemia, delayed developments and movement disorders. Studies have proved that when used in combination with folic acid, it can prevent heart diseases.

Vitamin B6, which active form pyridoxal phosphate or otherwise known as PLP and pyridoxal-5’-phosphate, is a prosthetic group of tightly bound cofactors responsible for many reactions within the amino acid metabolism and the manipulation of the enzymatic reaction behind the release of glucose from glycogen. Vitamin B6 generally comprises three organic compounds, pyridoxine, pyridoxal and pyridoxamine, all of them heterocyclic organic compounds that are converted in the human body to the active pyridoxal phosphate form. There are three reactions attributed to PLP, namely transamination, alpha elimination and beta elimination. Over one hundred and forty enzyme activities depend on pyridoxal phosphate, neurotransmitter and histamine synthesis, synthesis and function of hemoglobin, and gene expression. As a cofactor, PLP is active in amino acid metabolism, glucogenesis and lipid metabolism.

Zinc, a mineral needed for cell division and repair as well as proper functioning of the immune system. Zinc is also an important antioxidant that neutralizes free radicals in the body.